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KMID : 0368420090520010041
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Journal of Plant Biology
2009 Volume.52 No. 1 p.41 ~ p.48
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Flavonoid O-Diglucosyltransferase from Rice: Molecular Cloning and Characterization
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Kim Bong-Gyu
Kim Na-Yeon
Kim Jeong-Ho
Akimitsu Kazuya
Chong You-Hoon
Ahn Joong-Hoon
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Abstract
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Among more than 100 rice uridine diphosphate glycosyltransferases (UGTs), OsUGT-3 was selected as a candidate for producing flavonoid O-diglycosyltransferases based on phylogenetic analysis and molecular docking. This gene was functionally expressed in Escherichia coli. Analysis of kaempferol, luteolin, quercetin, and tricin reaction products using liquid chromatography-mass spectrometry revealed that these were diglucosylated. The glucosylation positions of kaempferol, which was the best substrate, were determined to be the 3- and 7-hydroxyl groups. This is the first flavonoid O-diglucosyltransferase described from rice.
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KEYWORD
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Flavonoids, Flavonoid O-diglucoside, Glycosyltransferase, Oryza sativa, UGT
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